Monocyte-derived Nucleic Acids and Related Compositions and Methods
AssigneeMerck Sharp & Dohme Corp.
InventorsELIZABETH BATES, NATHALIE FOURNIER, LIONEL CHALUS, PIERRE GARRONE
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Abstract
Nucleic acids encoding various monocyte-derived proteins and related compositions, including purified proteins and specific antibodies are described. Methods of using such composition are also provided.
Claims16
An isolated polypeptide comprising an amino acid sequence derived from SEQ ID NO:
- 2, 4, 6, 8 or 10.
An isolated nucleic acid comprising a nucleotide sequence encoding an amino acid sequence derived from SEQ ID NO:
- 2, 4, 6, 8 or 10.
A method for detecting a specific nucleic acid sequence in a sample, said method comprising the steps of:
- (i) contacting a sample suspected to contain a specific nucleic acid sequence with a probe comprising a nucleic acid sequence comprising at least 8 consecutive nucleotides selected from SEQ ID NO:
- 1, 3, 5, 7, or 9 under conditions in which a hybrid can form between said probe and the specific nucleic acid in said sample;
- and
- (ii) detecting any hybrid formed in step (i), wherein detection of said hybrid indicates the presence of the specific nucleic acid sequence in said sample.
A method for detecting a specific antigenic component in a sample, said method comprising the steps of:
- (i) contacting a sample suspected to contain a specific antigenic component encoded by an amino acid sequence derived from SEQ ID NO:
- 2, 4, 6, 8, or 10 with an antibody specific for said component, under conditions in which a stable antigen-antibody complex can form between said antibody and said antigenic component in said sample;
- and
- (ii) detecting any antigen-antibody complex formed in step (i), wherein detection of an antigen-antibody complex indicates the presence of said antigenic component in said sample.
A method of screening for candidate therapeutic agents comprising:
- selecting as a target sequence a polypeptide having an amino acid sequence derived from SEQ ID NO:
- 2, 4, 6, 8 or 10;
- contacting a test compound with said target sequence;
- and
- selecting as candidate therapeutic agent those test compounds which bind to the target sequence.
Dependencies are inferred from the claim text.