Method for preparing a hydrocarbon
AssigneeShell Oil Company
InventorsThomas Paul HOWARD, Sabine MIDDELHAUFE, Dagmara KOLAK, Stephen J. AVES, John LOVE, David PARKER, George Robert LEE
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Abstract
A method for preparing a hydrocarbon comprising contacting a fatty acid substrate with at least one fatty acid reductase and at least one fatty aldehyde synthetase and at least one fatty acyl transferase, wherein the fatty acid substrate is a fatty acid, a fatty acyl-ACP, or a fatty acyl-CoA or a mixture of any of these, to obtain a fatty aldehyde; and contacting the fatty aldehyde with at least one aldehyde decarbonylase enzyme.
Claims64
A method for preparing a hydrocarbon comprising:
- obtaining a fatty acid aldehyde by contacting a fatty acid substrate with at least one fatty acid reductase, at least one fatty aldehyde synthetase, and at least one fatty acyl transferase, wherein the fatty acid substrate is selected from the group consisting of a fatty acid, a fatty acyl-ACP, a fatty acyl-CoA and any combination thereof;
- and
- obtaining a hydrocarbon by contacting the fatty aldehyde with at least one aldehyde decarbonylase.
A recombinant host cell comprising a fatty acid reductase and a fatty aldehyde synthetase and a fatty acyl transferase.
An expression vector comprising at least one polynucleotide comprising a sequence selected from the group consisting of SEQ ID NO:11 to SEQ ID NO:25, a complement thereof, and a sequence which will hybridize under stringent conditions to any such polynucleotide sequence.
Dependencies are inferred from the claim text.