Filamentous Fungi and Methods for Producing Isoprenoids
AssigneeNovozymes A/S
InventorThomas Hohn
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Abstract
The present invention relates to the production of a isoprenoid products from a lignocellulosic feedstock. Specifically at least triple mutant of filamentous fungi having the isoprenoid pathway results in production of isoprenoid products in commercial quantities. One embodiment of the invention relates to producing the isoprenoid products at the site of the lignocellulosic feedstock to reduce costs of shipping the feedstock.
Claims31
A mutant isoprenoid producing filamentous fungus having the trichothecenes pathway comprising:
- a) a disrupted Tri5 gene, or a mutant Tri5 gene having low trichodiene synthase production;
- and
- b) a modified Tri6 gene, a modified Tri10 gene, or a modified gene encoding a terpene synthase, the gene modified to increase production of the gene product;
- wherein the mutant filamentous fungus produces at least 10% more isoprenoid product than the parent filamentous fungal cell when cultured under the same conditions.
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A mutant isoprenoid producing filamentous fungus having the trichothecenes pathway comprising:
- a) a modified Tri6 gene, a modified Tri10 gene, or a modified gene encoding a terpene synthase, the gene modified to increase production of the gene product;
- and
- b) the presence of a Tri5 inhibitor sufficient to inhibit at least a portion of the Tri5 gene product;
- wherein the mutant filamentous fungus produces at least 10 percent more isoprenoid than the parent filamentous fungal cell when cultured under the same conditions.
18-22. (canceled)
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Dependencies are inferred from the claim text.