Diterpene Synthases And Method For Producing Diterpenoids
AssigneeThe University of British Columbia
InventorsJoerg Bohlmann, Philipp Zerbe
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Abstract
Provided herein are diterpene synthases (diTPS) and methods for producing diterpenoids. Also provided herein are nucleic acid sequences encoding diTPS, diTPS amino acid sequences, diTPS proteins, vectors, cells, transgenic organisms, uses, compositions, methods, processes, and kits thereof.
Claims60
An isolated nucleic acid molecule, comprising a sequence of nucleotides encoding a cis-abienol synthase (CAS) polypeptide selected from among:
- a) the polypeptide whose sequence is set forth in one of SEQ ID NOS:7, 50 and 55;
- b) an active fragment of the polypeptide of a);
- and
- c) a polypeptide having a sequence of amino acids that has at least 85% sequence identity with a polypeptide of a) or b),
- wherein the encoded polypeptide or active fragment catalyzes the formation of cis-abienol from geranylgeranyl diphosphate (GGPP).
A cis-abienol synthase (CAS) polypeptide selected from among:
- a) the polypeptide whose sequence is set forth in one of SEQ ID NOS:7, 50 and 55;
- b) an active fragment of the polypeptide of a);
- and
- c) a polypeptide having a sequence of amino acids that has at least 85% sequence identity with a polypeptide of a) or b),
- wherein the polypeptide or active fragment catalyzes the formation of cis-abienol from geranylgeranyl diphosphate (GGPP).
An isolated nucleic acid molecule, comprising a sequence of nucleotides encoding a levopimaradiene/abietadiene synthase (LAS) polypeptide selected from among:
- a) the polypeptide whose sequence is set forth in one of SEQ ID NOS:
- 1 and 47;
- b) an active fragment of the polypeptide of a);
- and
- c) a polypeptide having a sequence of amino acids that exhibits greater than 99% with a polypeptide of a) or b),
- wherein the encoded polypeptide or active fragment catalyzes the formation of 13-hydroxy-8(14)-abietene from geranylgeranyl diphosphate (GGPP).
A levopimaradiene/abietadiene synthase (LAS) polypeptide selected from among:
- a) the polypeptide whose sequence is set forth in one of SEQ ID NOS:
- 1 and 47;
- b) an active fragment of the polypeptide of a);
- and
- c) a polypeptide having a sequence of amino acids that exhibits greater than 99% with a polypeptide of a) or b),
- wherein the polypeptide or active fragment catalyzes the formation of 13-hydroxy-8(14)-abietene from geranylgeranyl diphosphate (GGPP).
An isolated nucleic acid molecule, comprising a sequence of nucleotides encoding an isopimaradiene synthase (Iso) polypeptide selected from among:
- a) the polypeptide whose sequence is set forth in one of SEQ ID NOS:
- 3 and 48;
- b) an active fragment of the polypeptide of a);
- and
- c) a polypeptide having a sequence of amino acids that has at least 95% sequence with a polypeptide of a) or b),
- wherein the encoded polypeptide or active fragment catalyzes the formation of isopimaradiene from geranylgeranyl diphosphate (GGPP).
An isopimaradiene synthase (Iso) polypeptide selected from among:
- a) the polypeptide whose sequence is set forth in one of SEQ ID NOS:
- 3 and 48;
- b) an active fragment of the polypeptide of a);
- and
- c) a polypeptide having a sequence of amino acids that has at least 95% sequence with a polypeptide of a) or b),
- wherein the polypeptide or active fragment catalyzes the formation of isopimaradiene from geranylgeranyl diphosphate (GGPP).
Dependencies are inferred from the claim text.