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US 2013/0217086 A1Published application
Modified microorganism for production of 1,4-butanediol
AssigneeSamsung Electronics Co., Ltd.
InventorsYoung Min LEE, Hyun Min KOO, Jae Young KIM, Jin Woo KIM, Jae Chan PARK, Hwa Young CHO, Joon Song PARK, Pyung Cheon LEE
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Abstract
A modified microorganism for production of 1,4-butanediol, an expression vector, and a method of producing 1,4-butanediol using the modified microorganism are provided. The method can be useful in producing 1,4-butanediol using a biological production process.
Claims22
A modified microorganism for producing 1,4-butanediol, wherein the modified microorganism
- converts α-ketoglutarate or succinate into 4-hydroxybutyryl-CoA;
- converts the 4-hydroxybutyryl-CoA into 1,4-butanediol, and
- comprises at least one heterologous polynucleotide sequence selected from the group consisting of adh1, yiaY, adh4, adhB, mdh, eutG, fucO, dhaT, aldA, eutE, adhE1, adhE2 adh2, and polynucleotide sequences encoding the gene products thereof.
An expression vector comprising:
- a polynucleotide comprising a nucleotide sequence encoding an enzyme that converts α-ketoglutarate or succinate into 4-hydroxybutyryl-CoA;
- and
- a polynucleotide comprising a nucleotide sequence encoding an enzyme that converts 4-hydroxybutyryl-CoA into 1,4-butanediol,
- wherein the nucleotide sequence encoding an enzyme that converts 4-hydroxybutyryl-CoA into 1,4-butanediol is at least one selected from the group consisting of adh1, yiaY, adh4, adhB, mdh, eutG, fucO, dhaT, aldA, eutE, adhE1, adhE2, adh2, and polynucleotide sequences encoding the gene products thereof.
1 more element
Dependencies are inferred from the claim text.