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AssigneeMonsanto Technology LLC
InventorsJudith C. Donovan, William P. Donovan, James T. Engleman, Thomas M. Malvar, John W. Pitkin
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The present invention relates to the isolation and characterization of nucleotide sequences encoding novel insecticidal proteins secreted into the extracellular space from Bacillus thuringiensis and related strains. The proteins are isolated from culture supernatants of Bacillus thuringiensis and related strains and display insecticidal activity against lepidopteran insects including European corn borer (ECB), tobacco budworm (TBW) and diamondback moth (DBM). Insecticidal proteins encoded by nucleotide sequences that hybridize under stringent conditions to the isolated and characterized nucleotide sequences are disclosed. Methods are disclosed for making and using transgenic cells and plants comprising the novel nucleotide sequence of the invention.
A recombinant polynucleotide which encodes a Bacillus thuringiensis insecticidal toxin protein or insecticidal fragment thereof, active against a lepidopteran insect pest, wherein said insecticidal toxin protein comprises a polypeptide sequence that has at least about 80% sequence identity to SEQ ID NO:6 (TIC402).
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An insecticidal protein active against Lepidopteran insects, said protein comprising an amino acid sequence that has at least about 80% sequence identity to SEQ ID NO:6 (TIC402).
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A method for expressing a lepidopteran-active toxin protein in a plant, comprising the steps of:
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Dependencies are inferred from the claim text.