Polypeptides Having Hemicellulolytic Activity And Polynucleotides Encoding Same
AssigneesNovoyzmes A/S, Novozymes, Inc.
InventorsNikolaj Spodsberg, Mary Stringer, Hanshu Ding
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Abstract
The present invention relates to isolated polypeptides having hemicellulolytic activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.
Claims20
An isolated polypeptide having hemicellulolytic activity, selected from the group consisting of:
- (a) a polypeptide having at least 60% sequence identity to the mature polypeptide of SEQ ID NO:
- 20;
- at least 70% sequence identity to the mature polypeptide of SEQ ID NO:
- 22 or SEQ ID NO:
- 24;
- at least 75% sequence identity to the mature polypeptide of SEQ ID NO:
- 2, SEQ ID NO:
- 6, SEQ ID NO:
- 8, SEQ ID NO:
- 10, or SEQ ID NO:
- 18;
- at least 80% sequence identity to the mature polypeptide of SEQ ID NO:
- 16;
- or at least 85% sequence identity to the mature polypeptide of SEQ ID NO:
- 4, SEQ ID NO:
- 12, or SEQ ID NO:
- 14;
- (b) a polypeptide encoded by a polynucleotide that hybridizes under at least high stringency conditions with (i) the mature polypeptide coding sequence of SEQ ID NO:
- 1, SEQ ID NO:
- 3, SEQ ID NO:
- 5, SEQ ID NO:
- 9, SEQ ID NO:
- 11, SEQ ID NO:
- 13, SEQ ID NO:
- 15, SEQ ID NO:
- 17, SEQ ID NO:
- 19, SEQ ID NO:
- 21, or SEQ ID NO:
- 23, (ii) the cDNA thereof, or (iii) the full-length complement of (i) or (ii);
- (c) a polypeptide encoded by a polynucleotide having at least 60% sequence identity to the mature polypeptide coding sequence of SEQ ID NO:
- 19 or the cDNA sequence thereof;
- at least 70% sequence identity to the mature polypeptide coding sequence of SEQ ID NO:
- 1, SEQ ID NO:
- 21, or SEQ ID NO:
- 23, or the cDNA sequence thereof;
- at least 75% sequence identity to the mature polypeptide coding sequence of SEQ ID NO:
- 5, SEQ ID NO:
- 7, SEQ ID NO:
- 9, or SEQ ID NO:
- 17, or the cDNA sequence thereof;
- at least 80% sequence identity to the mature polypeptide coding sequence of SEQ ID NO:
- 15 or the cDNA sequence thereof;
- or at least 85% sequence identity to the mature polypeptide coding sequence of SEQ ID NO:
- 3, SEQ ID NO:
- 11, or SEQ ID NO:
- 13, or the cDNA sequence thereof;
- and
- (d) a fragment of the polypeptide of (a), (b), (c), or (d) that has hemicellulolytic activity.
A recombinant host cell comprising the polynucleotide of claim 10 operably linked to one or more control sequences that direct the production of the polypeptide.
An isolated polynucleotide encoding a signal peptide comprising or consisting of amino acids 1 to 18 of SEQ ID NO:
- 2, amino acids 1 to 16 of SEQ ID NO:
- 4, amino acids 1 to 18 of SEQ ID NO:
- 6, amino acids 1 to 19 of SEQ ID NO:
- 8, amino acids 1 to 20 of SEQ ID NO:
- 10, amino acids 1 to 26 of SEQ ID NO:
- 12, amino acids 1 to 23 of SEQ ID NO:
- 14, amino acids 1 to 28 of SEQ ID NO:
- 16, amino acids 1 to 20 of SEQ ID NO:
- 18, amino acids 1 to 18 of SEQ ID NO:
- 20, amino acids 1 to 20 of SEQ ID NO:
- 22, or amino acids 1 to 21 of SEQ ID NO:
- 24.
Dependencies are inferred from the claim text.