Process for transfecting plants
AssigneeNomad Bioscience GmbH
InventorsAnatoli Giritch, Yuri Symonenko, Simone Hahn, Doreen Tiede, Anton Shvarts, Patrick Roemer, Yuri Gleba
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Abstract
A process of transfecting a plant, comprising spraying parts of said plant with an aqueous suspension containing cells of an Agrobacterium strain and at least one abrasive suspended in said suspension, said Agrobacterium strain comprising a DNA molecule comprising a nucleic acid construct containing a DNA sequence of interest to be transfected into the plant.
Claims15
A process of transfecting a plant, comprising spraying aerial parts of said plant with an aqueous suspension containing cells of an Agrobacterium strain and at least one abrasive suspended in said suspension, said Agrobacterium strain comprising a DNA molecule comprising a nucleic acid construct containing a DNA sequence of interest to be transfected into cells of the plant.
A process of generating or altering a trait in a plant, comprising
- (i) growing said plant up to a desired growth state;
- (ii) expressing, in said plant, a protein or an RNA capable of generating or altering said trait, comprising spraying aerial parts of said plant with an aqueous suspension containing cells of an Agrobacterium strain and at least one abrasive suspended in said suspension,
- said Agrobacterium strain comprising a DNA molecule comprising a nucleic acid construct containing a DNA sequence of interest, said DNA sequence of interest encoding said protein or said RNA.
A process of producing a protein of interest in a plant, comprising
- (i) growing said plant up to a desired growth state;
- (ii) expressing, in said plant, said protein of interest, comprising spraying aerial parts of said plant with an aqueous suspension containing cells of an Agrobacterium strain and at least one abrasive suspended in said suspension,
- said Agrobacterium strain comprising a DNA molecule comprising a nucleic acid construct containing a DNA sequence of interest, said DNA sequence of interest encoding said protein of interest.
A process of protecting crop plants on a field from a pest, comprising
- (i) growing said plants in soil of said field;
- (ii) determining, in a desired growth state of said plants, infestation of at least one of said plants by a pest;
- (iii) expressing, in said plants, a protein or an RNA that is detrimental to the pest determined in the previous step, comprising spraying aerial parts of said plants with an aqueous suspension containing cells of an Agrobacterium strain and at least one abrasive suspended in said suspension,
- said Agrobacterium strain comprising a DNA molecule comprising a nucleic acid construct containing a DNA sequence of interest operably linked to a promoter, said DNA sequence of interest encoding said protein or said RNA.
Aqueous suspension containing cells of an Agrobacterium strain and at least one abrasive suspended in said suspension, wherein said aqueous suspension contains said cells of said Agrobacterium strain in a concentration of at most 4.4·107, preferably of at most 1.1·107, preferably of at most 4.4·106, more preferably of at most 1.1·106 cfu/ml of said suspension;
- said Agrobacterium strain comprising a heterologous DNA molecule comprising a nucleic acid construct containing a heterologous DNA sequence of interest that may be operably linked to a promoter;
- said suspension optionally further comprising a preferably non-ionic wetting agent such as an organosilicone surfactant.
Dependencies are inferred from the claim text.