Enzyme directed oil biosynthesis in microalgae
AssigneeBoard of Trustees of Michigan State University
InventorsChristoph Benning, Rachel Miller, Eric R. Moellering
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Abstract
The present invention is related to biosynthetic oil compositions and methods of making thereof. In some embodiments, the invention relates to the use of endogenous enzymes in plants capable of synthesizing oil. In preferred embodiments, said plants are algae. In further embodiments, said algae are from the family Chlamydomonas, Nannochloropsis, Dunaliella, Chiarella and Scenedesmus. In still further embodiments, said endogenous enzymes are diacylglycerol acyltransferases.
Claims17
A method comprising:
- a) providing:
- i) algae, and
- ii) a vector comprising a nucleic acid encoding a diacylglycerol acyltransferase operably linked to a promoter, wherein said nucleic acid has greater than 90% sequence identity to a sequence is selected from the group consisting of SEQ ID NO:
- 5, SEQ ID NO:
- 8 and SEQ ID NO:
- 11;
- b) transfecting said algae with said vector under conditions such that said diacylglycerol acyltransferase is expressed.
Algae transfected with a nucleic acid encoding a diacylglycerol acyltransferase operably linked to a promoter, wherein said nucleic acid has greater than 90% sequence identity to a sequence selected from the group consisting of SEQ ID NO:
- 5, SEQ ID NO:
- 8 and SEQ ID NO:
- 11.
A non-algae plant tissue comprising a nucleic acid encoding an algae diacylglycerol acyltransferase operably linked to a promoter, wherein said nucleic acid has greater than 90% sequence identity to a sequence selected from the group consisting of SEQ ID NO:
- 5, SEQ ID NO:
- 8 and SEQ ID NO:
- 11.
A method comprising:
- a) providing:
- i) a rutabaga, and
- ii) a vector comprising a nucleic acid encoding a diacylglycerol acyltransferase operably linked to a promoter, wherein said nucleic acid has greater than 90% sequence identity to a sequence selected from the group consisting of SEQ ID NO:
- 5, SEQ ID NO:
- 8 and SEQ ID NO:
- 11;
- b) transfecting said rutabaga with said vector under conditions such that said diacylglycerol acyltransferase is expressed.
A rutabaga plant tissue comprising a nucleic acid encoding a diacylglycerol acyltransferase operably linked to a promoter, wherein said nucleic acid has greater than 90% sequence identity to a sequence selected from the group consisting of SEQ ID NO:
- 5, SEQ ID NO:
- 8 and SEQ ID NO:
- 11.
A vector, comprising a nucleic acid encoding an algae diacylglycerol acyltransferase, wherein said nucleic acid is operably linked to a promoter, and wherein said nucleic acid has greater than 90% sequence identity to a sequence selected from the group consisting of SEQ ID NO:
- 5, SEQ ID NO:
- 8 and SEQ ID NO:
- 11.
Dependencies are inferred from the claim text.