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US 2013/0212722 A1Published application
Transgenic animals with customizable traits
AssigneeMice With Horns, LLC
InventorJAMES WEST
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Abstract
Disclosed are materials and methods for creating customizable traits in animals. In the demonstration of the principle of the subject invention, a keratin-14 specific promoter is used with, red fluorescent protein in the loxp cassette, dominant black (ΔG23) beta defensin 103 in the pigment cassette, and an SV40 (with intron) polyadenylation sequence. When Cre recombinase (or HTNCre) is applied to the animal's skin in a carrier base (e.g., lipid bilayers), fur is permanently genetically modified to turn black in the shape in which it was applied.
Claims20
A cell of a transgenic non-human animal, wherein the cell comprises:
- an exogenous nucleic acid molecule operably linked to a promoter, wherein the expression of the exogenous nucleic acid molecule is under the control of an inducible expression system that requires the presence of an inducing agent to activate expression;
- and
- wherein the expression of the exogenous nucleic acid molecule is inhibited in the absence of the inducing agent;
- wherein the exogenous nucleic acid molecule is selected from:
- a nucleic acid molecule encoding a protein selected from pigment proteins;
- proteins involved in the synthesis and/or transport of biological pigments;
- proteins involved in skin texture;
- proteins involved in the fur texture and/or length;
- and proteins involved in the texture, structural strength, and/or length of nail, claw, and/or horn;
- and
- an inhibitory RNA coding sequence that interferes with the expression of a nucleic acid molecule encoding a protein selected from pigment proteins;
- proteins involved in the synthesis and/or transport of biological pigments;
- proteins involved in skin texture;
- proteins involved in the fur texture and/or length;
- and proteins involved in the texture, structural strength, and/or length of nail, claw, and/or horn.
12 more elements
Dependencies are inferred from the claim text.