Synthesis of nanotopographic biomimetic membranes for tissue culture, engineering and prosthetics applications

A nanostructured membrane comprising:

• a) a substrate with a silanized surface;
• and
• b) a crosslinked multilayered membrane contacting the silanized surface and comprising a first polyelectrolyte and a second polyelectrolyte;
• wherein the crosslinked membrane is covalently linked to the silanized surface, and wherein the crosslinked membrane comprises pores ranging from about 50 nanometers to about 1.5 micrometers.

A method for preparing a compliant nanostructured surface, the method comprising:

• a) contacting a surface with (3-acryloxypropyl)-trichlorosilane;
• b) forming an aqueous film between the surface and a nanostructured mold, wherein the film comprises polyethylene glycol diacrylate and a photoinitiator, to form an imprinted uncured polymer substrate;
• and
• c) exposing the imprinted uncured polymer substrate to ultraviolet light to form the compliant surface.