Back to results
US 2013/0211006 A1Published application
Conjugated Polymeric Particle and Method of Making Same
AssigneeLife Technologies Corporation
InventorsSteven Menchen, Alan Blanchard, Luisa Andruzzi, Shaheer Khan, Dmitriy Gremyachinskiy, Alfred Lui, Craig Stolarczyk, Tanya Sokolsky
Life of this patent
Estimating the term…
Abstract
A method of conjugating a substrate includes exchanging a counter ion associated with a biomolecule with a lipophilic counter ion to form a biomolecule complex, dispersing the biomolecule complex in a nonaqueous solvent, and coupling the biomolecule complex to a substrate in the presence of the nonaqueous solvent.
Claims20
A method of conjugating a substrate, the method comprising:
- exchanging a counter ion associated with a biomolecule with a lipophilic counter ion to form a biomolecule complex;
- dispersing the biomolecule complex in a nonaqueous solvent;
- and
- coupling the biomolecule complex to a substrate in the presence of the nonaqueous solvent.
1 more element
A method of conjugating a polymeric particle, the method comprising:
- exchanging a cationic counter ion associated with a polynucleotide with a lipophilic cationic counter ion to form a polynucleotide complex, the polynucleotide including a nucleophilic or an electrophilic reactive group;
- dispersing the polynucleotide complex in a non-reactive, nonaqueous solvent;
- and
- coupling the polynucleotide to the polymeric particle by nucleophilic or electrophilic substitution, the polymeric particle including an electrophilic group or a nucleophilic group.
1 more element
Dependencies are inferred from the claim text.