Using a novel high-throughput yeast cell-based screening platform to identify hmg-coa reductase inhibitors from natural products and the uses of such inhibitors thereof
AssigneeHong Kong Baptist University
InventorsWendy Wen Luan HSIAO, William Chi-Shing TAI, Zhi-Hong JIANG
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Abstract
Methods of screening, identifying and isolating HMG-CoAR inhibitors are disclosed herein. In particular, said method is useful for screening natural raw products. The present invention also provides potent HMG-CoAR inhibitors for treatment and prevention of diseases and conditions associated with elevated cholesterol and/or blood lipid levels.
Claims14
A method for screening to identify enzyme 3-hydroxy-3-methylglutaryl coenzyme A reductase (HMG-CoAR) inhibitors comprising:
- providing a yeast strain which lacks HMG1 and HMG2 genes but containing a HMG1 overexpression plasmid;
- contacting a test material to said yeast strain;
- measuring cell viability of said yeast strain;
- adding mevalonate, wherein the test material possesses HMG-CoAR inhibitory activity if the measured cell viability is reduced when the test material is introduced to the yeast strain without mevalonate but in the presence of mevalonate the cell viability is not reduced.
A composition for treating a disease and/or condition associated with elevated cholesterol and/or blood lipid level comprising an effective amount of a extract of Gynostemma pentaphyllum (Gp), compounds isolated from Gp, total saponins from Gp, fraction from total saponins from Gp, isolated saponins from Gp, a compound having Formula (I) or (II) or a combination thereof, wherein Formula (I) and Formula (II) are
A composition for preventing a disease and/ or condition associated with elevated cholesterol and/or blood lipid level comprising an effective amount of a extract of Gynostemma pentaphyllum (Gp), compounds isolated from Gp, total saponins from Gp, fraction from total saponins from Gp, isolated saponins from Gp , a compound having Formula (I) or (II), or a combination thereof, wherein Formula(I) and Formula (II) are
Dependencies are inferred from the claim text.