Peptides, constructs and uses therefor
AssigneeAuckland Uniservices Limited
InventorGeoffrey Wayne Krissansen
Life of this patent
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Abstract
The invention provides novel peptides and constructs. In addition the invention provides methods for carrying compounds across the cell membrane, for antagonizing or destroying X-protein of HBV, treatment and/or management of HBV infection, treatment and/or prevention of HCC, and degradation of a target protein.
Claims18
1-65. (canceled)
An isolated peptide consisting of a first segment having an amino acid sequence selected from the group consisting of:
- (SEQ ID NO:
- 1)
- LCLRP;
- (SEQ ID NO:
- 2)
- LCLRPVG;
- (SEQ ID NO:
- 90)
- LCLRPVGAESRGRPV;
- (SEQ ID NO:
- 6)
- LCLRPVGAESRGRPVSGPFG;
- (SEQ ID NO:
- 71)
- LCLRPVGAESRGRPVSGPF;
- (SEQ ID NO:
- 70)
- LCLRPVGAESRGRPVSGP;
- (SEQ ID NO:
- 69)
- LCLRPVGAESRGRPVSG;
- (SEQ ID NO:
- 68)
- LCLRPVGAESRGRPVS;
- (SEQ ID NO:
- 67)
- LCLRPVGAESRGRPV;
- (SEQ ID NO:
- 66)
- LCLRPVGAESRGRP;
- (SEQ ID NO:
- 65)
- LCLRPVGAESRG;
- (SEQ ID NO:
- 64)
- LCLRPVGAER;
- and
- (SEQ ID NO:
- 3)
- MAARLCCQLDPARDVLCLRP;
- or a functionally equivalent variant thereof;
- and, optionally, a second segment connected to the first segment and comprising one or more heterologous amino acids such that the isolated peptide is a fusion peptide.
An isolated peptide comprising the amino acid sequence MAARLCCQ (SEQ ID NO:
- 7), or a functionally equivalent variant thereof, excluding peptides consisting of the full length sequence of the Hepatitis B virus X-protein and a peptide consisting of the amino acid sequence MAARVCCQL.
A construct comprising a peptide comprising the amino acid sequence LCLRP (SEQ ID NO:
- 1) or the amino acid sequence MAARLCCQ (SEQ ID NO:
- 7), or a functionally equivalent variant of any one thereof and a compound desired to be delivered to a cell.
A method for increasing the cell membrane permeability of a compound, the method comprising connecting a peptide comprising the amino acid sequence LCLRP (SEQ ID NO:
- 1), MAARLCCQ (SEQ ID NO:
- 7), or a functionally equivalent variant thereof, to the compound.
A method for delivering a compound into a cell, the method comprising contacting the cell or a composition comprising the cell with a construct that includes the compound and a peptide comprising the amino acid sequence LCLRP (SEQ ID NO:
- 1) or the amino acid sequence MAARLCCQ (SEQ ID NO:
- 7), or a functionally equivalent variant of any one thereof;
- or a nucleic acid encoding the construct.
Dependencies are inferred from the claim text.