Immunoreactive antigens of mycoplasma heamofelis and diagnostic immunoassay
AssigneePurdue Research Foundation
InventorsJoanne Belle Messick, Andrea Pires Santos
Life of this patent
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Abstract
Compositions for use in diagnostic screens for Mycoplasma haemofelis are provided. In one embodiment an immunogenic peptide selected from SEQ ID NO: 1 to SEQ ID NO: 60, or a fragment thereof, is immobilized on a solid support and is used to detect the presence of Mycoplasma haemofelis antibodies in a patient bodily fluid. In accordance with one embodiment a method for detecting a Mycoplasma haemofelis infection in a feline species, is provided.
Claims28
A peptide complex comprising
- a peptide wherein said peptide comprises an amino acid sequence selected from SEQ ID NO:
- 1 through SEQ ID NO:
- 60, or a contiguous 8 amino acid fragment of an amino acid selected from SEQ ID NO:
- 1 through SEQ ID NO:
- 60;
- and
- a solid support, wherein said peptide is immobilized on said solid support.
A composition comprising
- two or more isolated peptides wherein each of said isolated peptides comprises a contiguous span of 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21 22, 23, 24, 25, 26, 27, 28, 29, 30 amino acids wherein said contiguous span shares at least 80% sequence identity with a contiguous sequence of the same length contained within a sequence selected from the group consisting of SEQ ID NO:
- 1 through SEQ ID NO:
- 60.
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A method of detecting a Mycoplasma haemofelis infection in a warm blooded vertebrate, said method comprising
- analyzing a bodily fluid from said vertebrate for the presence of antibodies that specifically bind to an amino acid sequence selected from SEQ ID NO:
- 1 to SEQ ID NO:
- 60.
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An isolated or purified nucleic acid sequence comprising:
- 1) a nucleic acid sequence selected from the group consisting of SEQ ID NO:
- 61 through SEQ ID NO:
- 121, or a fragment or complementary sequence thereof;
- 2) a nucleic acid sequence that hybridizes with a sequence selected from the group consisting of SEQ ID NO:
- 61 through SEQ ID NO:
- 121 or a fragment, or a complementary sequence thereof under a medium or high stringency condition.
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Dependencies are inferred from the claim text.