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US 2013/0210660 A1Published application
Method for Active Hybridization in Microarrays with Denaturing Function
AssigneeRobert Bosch GmbH
InventorsMichael Stumber, Martina Daub, Jochen Rupp
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Abstract
A method for active hybridization in microarrays includes pumping an already prepared sample through a denaturing unit with a microarray and then through a reaction region which is spatially separate from the denaturing unit. The denatured reactive sample components are hybridized in the reaction region.
Claims11
A method for active hybridization to at least one probe of a microarray, comprising:
- a. providing a sample liquid containing reactive constituents,
- b. introducing the sample liquid into a pump route comprising at least one pump unit, at least one temperature-controlled denaturation unit and at least one temperature-controlled reaction area which is spatially separated from the at least one temperature-controlled denaturation unit and has at least one microarray,
- c. pumping the sample liquid,
- d. denaturing the reactive constituents of the sample liquid in the at least one temperature-controlled denaturation unit and
- e. hybridizing the denatured constituents to at least one probe of the at least one microarray in the at least one temperature-controlled reaction area.
2 more elements
A device for carrying out a method for active hybridization comprising:
- a sample liquid containing reactive constituents;
- and
- a pump route configured to receive the sample liquid, the pump route including:
- at least one pump unit configured to pump the sample liquid;
- at least one temperature-controlled denaturation unit configured to denature the reactive constituents of the sample liquid;
- and
- at least one temperature-controlled reaction area which is spatially separated from the denaturation unit and has at least one microarray with at least one probe, the at least one temperature-controlled reaction area being configured to hybridize the denatured constituents to the at least one probe.
4 more elements
Dependencies are inferred from the claim text.