Molecular diagnostic screening assay
OwnerAndrew Watson, Darren Roy Link +2inventors
InventorsAndrew Watson, Darren Roy Link, Jeffrey Charles Olson, Michael Samuels
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Abstract
The invention generally relates to method for screening for a condition in a subject. In certain embodiments, methods of the invention involve obtaining a pool of nucleic acids from a sample, incubating the nucleic acids with first and second sets of binders, in which the first set binds uniquely to different regions of a target nucleic acid in the pool, the second set binds uniquely to different regions of a reference nucleic acid in the pool, and the first and second sets include different detectable labels, removing unbound binders, detecting the labels, and screening for a condition based upon results of the detecting step.
Claims50
A method for disease screening, the method comprising:
- obtaining nucleic acid from a sample;
- incubating the nucleic acid with first and second sets of binders, wherein members of the first set bind to different regions of a target nucleic acid, members of the second set bind to different regions of a reference nucleic acid, and members of the first set comprise a detectable label that is different than members of the second set;
- removing unbound binders;
- detecting the labels;
- and
- identifying a positive screen based upon differential presence of label between said first and second sets.
A method for screening for a condition in a subject, the method comprising:
- obtaining a pool of different nucleic acid from a sample;
- compartmentalizing the pool of nucleic acids into compartmentalized portions, the compartmentalized portions comprising, on average, either a first set of binders or a second set of binders, wherein the first and second sets comprise different detectable labels and the first and second sets bind to different nucleic acids in the pool;
- amplifying only binders in the compartmentalized portions that bound to the nucleic acid;
- detecting the labels;
- and
- screening for a condition based upon results of the detecting step.
Dependencies are inferred from the claim text.