Hemomosaic: high-throughput technique for rare cell detection in liquid samples by massively multiplexed pcr in a photolithographic matrix
AssigneesCalifornia Institute of Technology, University of Southern California
InventorsEmil P. Kartalov, Michael Kahn, Axel Scherer
Life of this patent
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Abstract
Described microfluidic technology focused on: 1) direct integration of microfluidic devices with solidified liquid tissue samples, 2) subordination of architectural and operational principles of microfluidic devices specific tissue structure and needs and/or 3) on-chip sample acquisition integrated with the detection measurement within the same device. In contrast to conventional methods of off-chip sample prep and subsequent insertion into a detection device, new applications are possible on solidified liquid or solid tissue samples, such as in situ PCR.
Claims19
An apparatus, comprising:
- a support;
- a cellular material mounted upon the support;
- and
- a photomask comprising at least one non-blocking region and at least one blocking region, wherein the photomask is placed over the cellular material such that each of the at least one blocking region is positioned to correspond to a region of interest of the cellular material.
A method for selectively isolating cellular material, comprising:
- positioning cellular material on a support;
- depositing a photosensitive material on the cellular material;
- applying a photomask comprising at least one non-blocking region and at least one blocking region onto the photosensitive material;
- and
- exposing photons to the photosensitive material through the at least one non-blocking region in order to define at least one access well, and wherein each of the at least one blocking region corresponds to a region of interest of the cellular material.
A method to detect analytes, comprising:
- solidifying liquid tissue;
- positioning the solidified liquid tissue on a support;
- depositing a photosensitive material on the solidified liquid tissue;
- applying a photomask comprising at least one non-blocking region and at least one blocking region onto the photosensitive material;
- exposing photons to the photosensitive material through the at least one non-blocking region in order to define at least one access well, and wherein each of the at least one blocking region corresponds to a region of interest of the solidified liquid tissue;
- filling the at least one access well with a reaction mixture comprising agents and components necessary for reaction to detect analytes;
- and
- performing the reaction simultaneously in the at least one access well, thereby detecting the analytes.
Dependencies are inferred from the claim text.