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US 2013/0210599 A1Published application
Centrifugal microfluidic disk and processing method using the same
AssigneeNational Taiwan University
InventorsAndrew Man Chung Wo, Chen-Lin Chen, Cheng-Wei Yang, Wei-Hao Lian
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Abstract
This invention provides a centrifugal microfluidic disk and methods for separating targets or cells and collecting the targets or cells in the centrifugal microfluidic disk by using the density gradient.
Claims20
A centrifugal microfluidic disk, at least comprising:
- a sample inlet for loading a blood or biological sample;
- a separation chamber, connected to the sample inlet, wherein the separation chamber contains an arc-shape portion;
- and
- a collection chamber, connected to the separation chamber.
1 more element
A centrifugal microfluidic disk, at least comprising:
- a sample inlet for loading a blood or biological sample;
- a separation chamber, connected to the sample inlet, wherein the separation chamber contains an arc-shape portion;
- and
- a settling chamber, connected to the separation chamber;
- a chemical inlet for loading a chemical, wherein the chemical inlet is connected to the collection chamber via a linking channel;
- and
- a collection chamber, connected to the separation chamber, wherein a first flow path of the sample is different to a second flow path of the chemical.
4 more elements
A method for separating a target from a sample, comprising:
- providing a microfluidic disk, wherein the microfluidic disk at least comprises:
- a sample inlet for loading a blood or biological sample;
- a separation chamber, connected to the sample inlet, wherein the separation chamber contains an arc-shape portion;
- and
- a collection chamber, connected to the separation chamber through at least a flow channel located in-between;
- introducing the sample into the sample inlet of the microfluidic disk;
- and
- spinning the microfluidic disk to drive the sample flowing outward through the separation chamber and the settling chamber, wherein the target is separated from the sample in the separation chamber and further flows into the collection chamber.
5 more elements
Dependencies are inferred from the claim text.