"thiamine pyrophosphate (tpp) riboswitch mutants producing vitamin b1 enriched food and feed crops"
AssigneesBen-Gurion University of the Negev, Yeda Research and Development Co. Ltd.
InventorsAsaph Aharoni, Samuel Bocobza, Michal Shapira
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Abstract
The present invention provides bioengineered organisms producing elevated levels of thiamine and/or thiamine derivatives. Particularly, the present invention discloses that modifying TPP-responsive riboswitch results in accumulation of thiamine and/or its derivatives.
Claims37
A thiamine producing bioengineered organism comprising a modified thiamine pyrophosphate (TPP)-responsive riboswitch having reduced affinity to TPP, wherein the organism produces elevated amounts of thiamine and/or derivatives thereof compared to a corresponding organism comprising an unmodified TPP-responsive riboswitch.
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A method for producing elevated amounts of thiamine and derivatives thereof by a thiamine-producing organism, the method comprising inserting at least one modification within a thiamine pyrophosphate (TPP)-responsive riboswitch polynucleotide sequence, wherein the modification results in reduced affinity of the riboswitch to TPP, thereby obtaining an organism producing elevated amounts of thiamine and/or derivatives thereof compared to a corresponding wild type organism.
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A method for producing elevated amounts of thiamine and derivatives thereof by a thiamine-producing organism, the method comprising transforming at least one organism cell with an expression cassette comprising a promoter, a polynucleotide encoding thiamine synthase and an untranslated sequence comprising modified riboswitch having reduced affinity to thiamine pyrophosphate (TPP), thereby obtaining an organism producing elevated amounts of thiamine and derivatives thereof compared to a corresponding wild type organism.
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Dependencies are inferred from the claim text.