Expression Of Positive Sense Single Stranded RNA Virus And Uses Thereof
AssigneeViracine Therapeutics Corporation
InventorArun K. Dhar
Life of this patent
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Abstract
The invention relates to the fields of viruses, vaccines and compounds and methods for expression. In particular, the invention includes methods and agents capable of producing quantities of a vaccine to a positive sense single stranded RNA (“(+)sense RNA”)virus.
Claims27
A baculovirus capable of infecting a facilitating host comprising a DNA sequence that codes for a functional positive sense single stranded RNA viral genome (“(+)sense RNA”) that is not capable of infecting said facilitating host, wherein the transcription of said DNA sequence is under the control of a single promoter.
-8. (canceled)
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A composition comprising a first virus capable of infecting a facilitating host comprising a first DNA sequence that codes for a (+)sense RNA viral genome or complement thereof that is not capable of infecting said facilitating host and a second DNA sequence coding for a baculovirus genome capable of infecting said facilitating host, wherein the expression of said first DNA sequence is under the control of a single promoter.
(canceled)
A method of amplifying a (+)sense RNA viral genome comprising
- a) infecting a facilitating host cell with virus comprising a DNA molecule comprising a first DNA sequence coding for a viral genome of said facilitating host cell and a second DNA sequence coding for a (+)sense RNA viral genome that does not infect said facilitating host cell, wherein said second DNA sequence is operably linked to only one promoter;
- b) obtaining the supernatant, cell lysate, or supernatant and cell lysate of said infected facilitating host cell;
- c) transducing a host cell of the (+)sense RNA virus with said supernatant, cell lysate, or supernatant and cell lysate.
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A vaccine comprising a (+)sense RNA viral genome produced by the method of
- a) infecting a facilitating host cell with a virus comprising a DNA molecule comprising a first DNA sequence coding for a viral genome of said facilitating host cell and a second DNA sequence coding for a (+)sense RNA viral genome that does not infect said facilitating host, wherein said second DNA sequence is operably linked to only one promoter;
- b) obtaining the supernatant, cell lysate, or supernatant and cell lysate of said facilitating host cell;
- and
- c) transducing a host cell of the (+)sense RNA with said supernatant, cell lysate, or supernatant and cell lysate, wherein the (+)sense RNA virus is inactivated or attenuated before use.
(canceled)
A vaccine comprising a (+)sense RNA virus that is not capable of infecting a facilitating host comprising a viral envelope comprising proteins derived from said facilitating host.
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Dependencies are inferred from the claim text.