Biochemical sensor

A biochemical sensor, comprising:

• a substrate, which has a printing surface, wherein the printing surface includes a reactive film, two electrode films, a conductive electrode film, and a referential electrode film, the conductive electrode film is arranged between the two electrode films, one end of the conductive electrode film, proximate to the reactive film, is connected to one end of the two electrode films, the referential electrode film and one of the two electrode films are arranged on the relative two sides of the printing surface, the reactive film has at least one first metallic reactive film, one non-metallic reactive film, and one second metallic reactive film, one end of the referential electrode film extends to establish contact with the first metallic reactive film, while each end of the two electrode films are in contact respectively with the non-metallic reactive film and the second metallic reactive film;
• an intermediate layer which has at least one notch in correspondence to the reactive film;
• and
• a cover which has at least one test slot in correspondence to the notch, the intermediate layer and the cover are attached sequentially to the substrate, the intermediate layer is arranged and inter-attached between the cover and the substrate.