Compositions and Methods for Culturing Microorganisms
AssigneeHelioBioSys, Inc.
InventorsRocco MANCINELLI, David SMERNOFF
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Abstract
This disclosure provides compositions and methods for culturing microorganisms. The disclosure includes mixtures of different microorganisms, especially mixtures of cyanobacteria with fermentative microorganisms. For example, methods and compositions related to co-cultures of yeast and cyanobacteria are provided. Also provided are feedstocks derived from cyanobacteria as well as methods of making such feedstocks and methods of culturing microorganisms in such feedstocks.
Claims29
-66. (canceled)
A culture comprising a consortia of cyanobacterial families, species and or strains and at least one fermentative microorganism selected from the group consisting of Saccharomycetes, Saccharomyces, Schizosaccharomyces, and fermentative bacteria.
A culture comprising fermentative microorganisms and medium derived from a culture of a consortia of cyanobacterial families, species and or strains, wherein said culture is substantially free of cyanobacteria.
A method of culturing microorganisms comprising:
- a. adding a population of fermentative microorganisms to a culture comprised of a consortia of cyanobacterial families, species and or strains, in order to obtain a microorganism co-culture, wherein the medium of said microorganism co-culture comprises a salt concentration greater than or equal to 0.08% (w/v);
- and
- b. culturing said microorganism co-culture under conditions to generate a fermentation product.
A method of culturing microorganisms comprising:
- a. removing cyanobacteria from a culture of cyanobacteria in order to obtain a feedstock medium substantially free of cyanobacteria;
- and
- b. culturing a population of fermentative microorganisms with said feedstock medium under conditions to generate a fermentation product.
A method of culturing microorganisms comprising:
- a. adding a population of fermentative microorganisms to a culture comprised of a consortia of cyanobacterial families, species and or strains, in order to obtain a microorganism co-culture;
- and
- b. culturing said microorganism co-culture under conditions to generate a fermentation product wherein said fermentative microorganisms receive growth media from products released by said cyanobacteria.
Dependencies are inferred from the claim text.