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US 2013/0174293 A1Published application
Colorimetric determination of the total oil content of a plant tissue sample using alkaline saponification
AssigneeDow AgroSciences LLC
InventorsThomas G. Patterson, Ted Freeman, Joshua A. Flook
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Abstract
This disclosure concerns plants, plant materials, and the oil characteristics thereof. In embodiments, compositions and methods for determining the total oil content of plant materials are provided. Such methods and compositions may in some embodiments allow for the characterization and screening of oil traits and sub-traits that are difficult or impossible to distinguish by conventional techniques.
Claims24
A method for determining the total oil content of a plant material, the method comprising:
- providing a sample of a plant material comprising oil;
- admixing the sample and a base, so as to saponify esters in the sample and thereby produce an amount of glycerol;
- detecting the amount of glycerol in the sample;
- calculating the amount of glycerol in the plant material;
- and
- determining the total oil content of the plant material.
3 more elements
A method for identifying a plant comprising at least one oil trait or sub-trait, the method comprising:
- providing a sample of an oil-containing plant material from a plant;
- admixing the sample and a base, so as to saponify esters in the sample and thereby produce an amount of glycerol;
- detecting the amount of glycerol in the sample;
- calculating the amount of glycerol in the plant material;
- determining the total oil content of the plant material, wherein a characteristic oil content is predictive of the presence of the at least one oil trait or sub-trait in the plant;
- and
- comparing the total oil content of the plant material with the characteristic oil content to determine whether the plant comprises the oil trait or sub-trait.
4 more elements
Dependencies are inferred from the claim text.