Optical detection method
AssigneeIndustrial Technology Research Institute
InventorsYung-Sung Lan, Chir-Weei Chang
Life of this patent
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Abstract
An method for detection of β-amyloid in aqueous humor, lens, and retina of eye or the deposit of the combination of αβ-crystallin and β-amyloid in lens is provided. A light is emitted to a testing area in the eye. The light frequency is selected according to an absorption spectrum of the test substance, and the frequency is equal or close to a resonant excitation frequency of one of the electronic molecular energy levels of the substance, so as to excite the substance to generate resonance-enhanced Raman effect or pre-resonance Raman effect to form a detection spectrum. The concentration of the substance could be estimated by a peak intensity of the detection spectrum.
Claims19
A method for detecting a concentration of a substance in an eye, the method comprising:
- selecting β-amyloid (Aβ) as the substance;
- emitting a light to a testing area in the eye, wherein a frequency of the light is selected according to an absorption spectrum of the selected substance, and the frequency is equal or close to a resonant excitation frequency of one of the electronic molecular energy levels of the substance, so as to excite the substance to generate resonance-enhanced Raman effect or pre-resonance Raman effect to form a detection spectrum;
- and
- receiving the detection spectrum and estimating the concentration of the substance according to a peak intensity of the detection spectrum.
A method for detecting a concentration of β-amyloid in a lens of an eye, the method comprising:
- emitting a light of a frequency to the lens, wherein the frequency of the light is equal or close to a resonant excitation frequency of one of the electronic molecular energy levels of a deposit of a combination of αβ-crystallin and β-amyloid, so as to excite the deposit of the combination of αβ-crystallin and β-amyloid to generate resonance-enhanced Raman effect or pre-resonance Raman effect to form a detection spectrum;
- and
- receiving the detection spectrum and estimating the concentration of the deposit of the combination of αβ-crystallin and β-amyloid according to a peak intensity of the detection spectrum, so as to obtain the concentration of β-amyloid in the lens.
A method for detecting a concentration of β-amyloid (Aβ) in an object, the method comprising:
- emitting a light to a testing area, wherein a frequency of the light is selected according to an absorption spectrum of β-amyloid (Aβ), and the frequency is equal or close to a resonant excitation frequency of one of the electronic molecular energy levels of β-amyloid (Aβ), so as to excite β-amyloid (Aβ) to generate resonance-enhanced Raman effect or pre-resonance Raman effect to form a detection spectrum;
- and
- receiving the detection spectrum and estimating the concentration of β-amyloid in the object according to a peak intensity of the detection spectrum.
Dependencies are inferred from the claim text.