Eicosapentaenoic acid-producing microorganisms, fatty acid compositions, and methods of making and uses thereof
AssigneeDsm IP Assets B.V.
InventorsJoseph W. PFEIFER III, Jon Milton Hansen, Jose R. Garcia, Xiao Daniel Dong, Paul Warren Behrens, Kirk E. Apt
Life of this patent
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Abstract
The present invention is directed to isolated microorganisms as well as strains and mutants thereof, biomasses, microbial oils, compositions, and cultures; methods of producing the microbial oils, biomasses, and mutants; and methods of using the isolated microorganisms, biomasses, and microbial oils.
Claims24
A method of making a biomass of a microorganism having fatty acids and a concentration of EPA, comprising:
- (a) fermenting the microorganism in a fermentor vessel to produce a biomass wherein the microorganism comprises a Thraustochytrid that produces a biomass having at least 3% EPA of the total weight of the fatty acids;
- and
- (b) attaining a desired EPA level in the biomass by (i) adjusting the amount of the biomass in the vessel and (ii) optionally adjusting the pressure on the biomass.
(canceled)
A method of making a biomass of a microorganism having fatty acids and a concentration of EPA, comprising:
- (a) fermenting the microorganism in a fermentor vessel to produce a fermentation broth and a biomass wherein the microorganism comprises a Thraustochytrid that produces a biomass having at least 3% EPA of the total weight of the fatty acids;
- and
- (b) attaining a desired EPA level in the biomass by (i) adjusting the temperature in the broth and (ii) optionally (ii) adjusting the pressure on the biomass.
A method of making a biomass of a microorganism having fatty acids and a concentration of EPA, comprising:
- (a) fermenting the microorganism in a fermentor vessel, comprising a gas, to produce a biomass wherein the microorganism comprises a Thraustochytrid that produces a biomass having at least 3% EPA of the total weight of the fatty acids;
- and
- (b) attaining a desired EPA level in the biomass by (i) adjusting the CO2 levels in the gas and (ii) optionally adjusting the pressure on the biomass.
A method of making a biomass of a microorganism having fatty acids and a concentration of EPA, comprising:
- (a) fermenting the microorganism in a fermentor vessel to produce a fermentation broth having an aqueous phase and biomass wherein the aqueous phase has a dissolved gas wherein the microorganism comprises a Thraustochytrid that produces a biomass having at least 3% EPA of the total weight of the fatty acids;
- and
- (b) attaining a desired EPA level in the biomass by (i) adjusting the dissolved CO2 to >2% of the dissolved gas, (ii) optionally adjusting the pressure on the biomass, and (iii) optionally adjusting the temperature in the broth.
6-7. (canceled)
A method of increasing the concentration of EPA in a biomass of a microorganism having fatty acids and a concentration of EPA, comprising:
- (a) fermenting the microorganism in a fermentor vessel, comprising a gas, to produce a biomass wherein the microorganism comprises a Thraustochytrid that produces a biomass having at least 3% EPA of the total weight of the fatty acids;
- and
- (b) supplementing the gas with CO2 sufficient to increase the concentration of the EPA in the biomass.
10-11. (canceled)
13-17. (canceled)
A method of increasing the concentration of EPA in a biomass of a microorganism having fatty acids and a concentration of EPA, comprising:
- (a) fermenting the microorganism in a fermentor vessel to produce a biomass;
- and
- (b) providing a pressure on the biomass of greater than or equal to 0.5 psi above atmospheric pressure for a time sufficient to increase the concentration of EPA in the biomass.
19-27. (canceled)
30-33. (canceled)
(canceled)
A method of making a biomass of a microorganism having fatty acids and a concentration of EPA, comprising:
- (a) fermenting the microorganism in a fermentor vessel, comprising a gas, to produce a biomass wherein the microorganism comprises a Thraustochytrid that produces a biomass having at least 3% EPA of the total weight of the fatty acids wherein the microorganism comprises a Thraustochytrid that produces a biomass having at least 3% EPA of the total weight of the fatty acids;
- and
- (b) supplementing the gas with CO2.
37-42. (canceled)
A method of making a biomass of a microorganism having increased concentration of EPA, comprising growing the microorganism in a culture medium comprising less than 0.1 mg/L vitamin B12 to produce a biomass.
44-54. (canceled)
A method of making a biomass of a microorganism having increased concentration of EPA, comprising growing the microorganism in a culture medium comprising less than 0.1 mg/L cobalt to produce a biomass.
56-66. (canceled)
Dependencies are inferred from the claim text.