Methods and compositions of preparation for proteome analysis
AssigneeCase Western Reserve University
InventorsMasaru Miyagi, Chandra Sekhar Rao Kadiyala
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Abstract
A method of extracting a polypeptide from a biological sample includes contacting the biological sample with an extraction reagent to form a solution of the biological sample and the extraction reagent. The extraction reagent includes perfluorooctanoic and can be used at a concentration effective to solubilize the polypeptides in the biological sample.
Claims22
A method of extracting a polypeptide from a biological sample, the method comprising:
- contacting the biological sample with an extraction reagent to form a solution of the biological sample and the extraction reagent, the extraction reagent being at a concentration effective to solubilize the polypeptides in the biological sample, the extraction reagent comprising perfluorooctanoic acid.
A method of extracting polypeptides from a biological sample, the method comprising:
- contacting the biological sample with an extraction reagent to form a solution of the biological sample and the extraction reagent, the extraction reagent being at a concentration effective to solubilize the polypeptides in the biological sample, the extraction reagent comprising up to about 2% w/v perfluorooctanoic acid;
- and
- removing the perfluorooctanoic acid from the solubilized polypeptides.
A method of extracting polypeptides from a biological sample, the method comprising:
- contacting the biological sample with an extraction reagent to form a solution of the biological sample and the extraction reagent, the extraction reagent being at a concentration effective to solubilize the polypeptides in the biological sample, the extraction reagent comprising up to about 2% w/v perfluorooctanoic acid;
- contacting the solution with a proteolytic enzyme to digest the polypeptides in the solution, the concentration of perfluorooctanoic acid in the solution during proteolytic digestion being about 0.1% w/v to about 0.5% w/v;
- and
- removing the perfluorooctanoic acid from the solubilized polypeptides.
Dependencies are inferred from the claim text.