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US 2013/0172289 A1Published application
Method for the preparation of sodium chondroitin sulphate
AssigneePierre Fabre Medicament
InventorsNadine Chomarat, Paul Grech, Guillermo Cueto, Edouardo Rodriguez, Jean-François Boe
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Abstract
The present invention concerns a method for obtaining sodium chondroitin sulphate from cartilage, notably of avian origin, as well as a sodium chondroitin sulphate preparation.
Claims17
Sodium chondroitin sulphate preparation comprising over 90% of sodium chondroitin sulphate which has a polydispersity index less than or equal to 1.47, measured by steric exclusion chromatography SEC-MALLS.
A method for the preparation of sodium chondroitin sulphate from cartilage comprising the following steps:
- a. Hydrolysis of the cartilage in aqueous medium,
- b. Thermal treatment of the hydrolysis between 90 and 100° C. for 2 to 10 hours,
- c. Separation of the sodium chondroitin sulphate solution from the hydrolysate,
- d. Flocculation of the sodium chondroitin sulphate solution and elimination of the flocculate,
- e. Purification of sodium chondroitin sulphate in the solution obtained in step d. by ultrafiltration,
- f. Treatment of the purified sodium chondroitin sulphate solution obtained in step e. by the addition of oxygenated water at a concentration of 0.02 to 0.2% by mass of oxygenated water with respect to total mass of the purified sodium chondroitin sulphate solution, and
- g. Precipitation of the sodium chondroitin sulphate preparation and isolation of the precipitate from the sodium chondroitin sulphate preparation.
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Dependencies are inferred from the claim text.