Methods for detecting plasticizers
AssigneeEast China University of Science and Technology
InventorsBangce Ye, Min Zhang
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Abstract
Nanoparticles having one or more attached sensing moieties including uridine 5′-triphosphate (UTP) and deoxythymidine 5′-triphosphate (dTTP), are disclosed herein. These nanoparticles can, for example, be used for detection of plasticizers, such as phthalates, in the sample. Methods, kits and apparatuses using these nanoparticles for detecting plasticizers in a sample are also disclosed herein.
Claims33
A method for detecting a plasticizer in a sample, the method comprising:
- providing a sample suspected of containing a plasticizer;
- providing a plurality of nanoparticles having one or more attached sensing moieties, wherein the sensing moiety comprises one or more uridine 5′-triphosphate (UTP) groups, uridine 5′-diphosphate (UDP) groups, uridine monophosphate (UMP) groups, 2′-deoxythymidine 5′-triphosphate (dTTP) groups, 2′-deoxythymidine 5′-diphosphate (dTDP) groups, or deoxythymidine 5′-monophosphate (dTMP) groups;
- contacting the sample with the plurality of nanoparticles in the presence of a crosslinker to form a mixture;
- maintaining the mixture under conditions allowing the crosslinker to bind the nanoparticles and any plasticizer present in the sample to form a nanoparticle aggregate;
- and
- detecting the nanoparticle aggregate.
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An apparatus for detecting a plasticizer in a sample, the apparatus comprising:
- at least one light source;
- and
- a receiver configured to receive at least a portion of the radiation emitted from the light source, wherein the receiver comprises a plurality of nanoparticles and a crosslinker, wherein the nanoparticles have one or more attached sensing moieties, wherein the sensing moiety comprises one or more uridine 5′-triphosphate (UTP) groups, uridine 5′-diphosphate (UDP) groups, uridine monophosphate (UMP) groups, 2′-deoxythymidine 5′-triphosphate (dTTP) groups, 2′-deoxythymidine 5′-diphosphate (dTDP) groups, or deoxythymidine 5′-monophosphate (dTMP) groups.
A kit for detecting a plasticizer in a sample, the kit comprising:
- a plurality of nanoparticles having one or more attached sensing moieties, wherein the sensing moiety comprises one or more uridine 5′-triphosphate (UTP) groups, uridine 5′-diphosphate (UDP) groups, uridine monophosphate (UMP) groups, 2′-deoxythymidine 5′-triphosphate (dTTP) groups, 2′-deoxythymidine 5′-diphosphate (dTDP) groups, or deoxythymidine 5′-monophosphate (dTMP) groups;
- and
- at least one crosslinker that binds the nanoparticles and the plasticizer.
A nanoparticle aggregate comprising:
- a plurality of nanoparticles having one or more attached sensing moieties, wherein the sensing moiety comprises one or more uridine 5′-triphosphate (UTP) groups, uridine 5′-diphosphate (UDP) groups, uridine monophosphate (UMP) groups, 2′-deoxythymidine 5′-triphosphate (dTTP) groups, 2′-deoxythymidine 5′-diphosphate (dTDP) groups, or deoxythymidine 5′-monophosphate (dTMP) groups;
- at least one plasticizer;
- and
- at least one crosslinker that binds the nanoparticles to the plasticizer.
Dependencies are inferred from the claim text.