Reversibly immortalized cells as well as methods relating hereto
AssigneeSanofi
InventorsYuri Ivashchenko, Thea Hennig
Life of this patent
Estimating the term…
Abstract
The present invention relates to methods of producing a reversibly immortalized cell, cells obtainable by the above method, eukaryotic cells comprising one or more nucleic acid(s) coding for one or more immortalizing protein(s) and methods of re-differentiating these cells.
Claims15
A method of producing a reversibly immortalized cell, the method comprising
- a) providing a eukaryotic cell,
- b) genetically modifying the cell of step a) to enable expression of one or more nucleic acid(s) coding for one or more immortalizing protein(s) in the cell;
- and
- c) expressing the one or more nucleic acid(s) in the cell of step b),
- wherein the one or more immortalizing protein(s) include(s) human papillomavirus (HPV) E6/E7 or a functionally active variant thereof, optionally in combination with (i) SV40 Large T antigen (TAG) or a functionally active variant thereof and/or (ii) telomerase catalytic subunit (TERT) or a functionally active variant thereof.
A method of producing a reversibly immortalized cell, the method comprising
- a) providing a eukaryotic cell,
- b) genetically modifying the cell of step a) to enable expression of one or more nucleic acid(s) coding for one or more immortalizing protein(s) in the cell;
- and
- c) expressing the one or more nucleic acid(s) in the cell of step b),
- wherein the at least one of the one or more immortalizing protein(s) is selected from the group consisting of human papillomavirus (HPV) E6/E7, SV40 Large T antigen and telomerase catalytic subunit (TERT), or a functionally active variant thereof,
- and wherein expression of one or more of the nucleic acid(s) coding for one or more immortalizing proteins is controlled by an EF1alpha promoter.
A eukaryotic cell comprising one or more nucleic acid(s) coding for one or more immortalizing protein(s), wherein the at least one of the one or more immortalizing protein(s) is selected from the group consisting of human papillomavirus (HPV) E6/E7, SV40 Large T antigen and telomerase catalytic subunit (TERT) and wherein expression of one or more of the nucleic acid(s) coding for one or more immortalizing protein(s) is controlled by an EF1alpha promoter.
Dependencies are inferred from the claim text.