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US 2013/0171721 A1Published application
Disruption of cell walls for enhanced lipid recovery
AssigneeAlliance for Sustainable Energy, LLC
InventorsEric P. KNOSHAUG, Bryon S. DONOHOE, Henri GERKEN, Lieve LAURENS, Stefanie Rose VAN WYCHEN
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Abstract
Presented herein are methods of using cell wall degrading enzymes for recovery of internal lipid bodies from biomass sources such as algae. Also provided are algal cells that express at least one exogenous gene encoding a cell wall degrading enzyme and methods for recovering lipids from the cells.
Claims20
A method for recovering lipids from a cell, comprising:
- a) contacting the cell with at least one cell wall degrading enzyme;
- and
- b) isolating lipids from the cell.
A method for recovering lipids from an algal cell, comprising:
- a) culturing the algal cell;
- b) inducing expression of a cell wall degrading enzyme in the algal cell;
- and
- c) extracting lipids from the algal cell by mixing the algal cell with a hexane/isopropanol solvent, separating out the solids, and recovering the lipids from the solvent.
1 more element
Dependencies are inferred from the claim text.