Puro-dhfr quadrifunctional marker and its use in protein production
AssigneeMerck Serono SA
InventorsMICHEL KOBR, PHILIPPE DUPRAZ
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Abstract
This invention relates to industrial production of proteins. More specifically, the invention relates to the res-DHFR surrogate marker, which corresponds to a fusion between DHFR and a protein conferring resistance to a toxic compound or conferring a metabolic advantage. The invention further relates to the use of res-DHFR for screening cells for high expression of a protein of interest. The invention is illustrated by the Puro-DHFR surrogate marker, which corresponds to a fusion between the puromycin N-acetyltransferase and dihydrofolate reductase (DHFR).
Claims10
An isolated polypeptide comprising a Puro-DHFR polypeptide comprising a fragment of puromycin N-acetyl transferase fused to a fragment of murine DHFR, wherein said Puro-DHFR polypeptide exhibits:
- (i) puromycin N-acetyl transferase activity;
- and (ii) dihydrofolate reductase activity.
Dependencies are inferred from the claim text.