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AssigneeAjinomoto Co., Inc.
InventorsYoshiya Gunji, Hisashi Yasueda, Shinichi Sugimoto, Nobuharu Tsujimoto, Megumi Shimaoka, Yuri Miyata, Manami Oba
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An L-amino acid is produced by culturing a Methylophilus bacterium which can grow by using methanol as the main carbon source and has L-amino acid-producing ability, for example, a Methylophilus bacterium in which dihydrodipicolinate synthase activity and aspartokinase activity are enhanced by transformation of cells with a DNA coding for dihydrodipicolinate synthase that is desensitized to feedback inhibition by L-lysine and a DNA coding for aspartokinase that is desensitized to feedback inhibition by L-lysine, or a Methylophilus bacterium which is casamino acid auxotrophic, in a medium containing methanol as a main carbon source, to produce and accumulate an L-amino acid in culture, and collecting the L-amino acid from the culture.
A method for producing L-lysine comprising culturing a Methylophilus bacterium in a medium containing methanol to produce and accumulate L-lysine in the medium, and collecting L-lysine from the medium, wherein said Methylophilus bacterium has been modified to have enhanced activities of dihydrodipicolinate synthase and aspartokinase by transformation with a DNA from Escherichia coli that encodes dihydrodipicolinate synthase resistant to feedback inhibition by L-lysine and with a DNA from Escherichia coli that encodes aspartokinase resistant to feedback inhibition by L-lysine.
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