Back to results
US 2013/0171697 A1Published application
Microfluidic device comprising rotatable disc-type body, and methods of separating target material and amplifying nucleic acid using the same
AssigneeSamsung Electronics Co., Ltd.
InventorsJong-myeon PARK, Myoung-soon KIM, Ye-ryoung YONG
Life of this patent
Estimating the term…
Abstract
A microfluidic device for controlling a flow of a fluid using centrifugal and rotational force based on a rotatable disc-type body, a method of separating a target material or performing emulsion nucleic acid amplification using the microfluidic device.
Claims24
A microfluidic device for separating a target material from a mixture of materials having different densities using a centrifugal force and a rotational force, the device comprising:
- a rotatable disc-type body having a center;
- a first chamber disposed in or on the body and spaced apart from the center in a centrifugal force direction;
- a second chamber disposed in or on the body and spaced apart from the first chamber in the centrifugal force direction and in fluid communication with the first chamber, the second chamber comprising an upper outlet and a lower outlet spaced apart from the upper outlet in the centrifugal force direction;
- and
- a plurality of third chambers disposed in or on the body, wherein each of the upper outlet and the lower outlet of the second chamber is in fluid communication with at least one of the third chambers.
2 more elements
A method for amplifying a nucleic acid, the method comprising:
- providing a microfluidic device of claim 23;
- introducing at least one nucleic acid amplification reagent to at least one of the plurality of first chambers of the device, and oil to at least one other of the plurality of first chambers of the device,
- introducing a sample including a template nucleic acid to a second chamber of the device;
- and
- rotating the body of the device, thereby transferring the reagent from one first chamber to the second chamber and mixing the reagent with the sample;
- and transferring the oil from another first chamber to the second chamber and forming an emulsion in which the sample is surrounded by the oil, wherein the body of the device is optionally rotated sequentially in a clockwise and counterclockwise direction;
- amplifying a nucleic acid of the sample in the emulsion by heating and/or cooling the second chamber;
- centrifuging a resultant by continuously rotating the body;
- and
- separating products of the amplification contained in a supernatant or a sediment via an upper outlet or a lower outlet of the second chamber.
7 more elements
Dependencies are inferred from the claim text.