Human Anti-B7RP1 Neutralizing Antibodies
AssigneeAmgen Inc.
InventorsGerald Siu, Wenyan Shen, Steven K. Yoshinaga, Haichun Huang
Life of this patent
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Abstract
This invention provides antibodies that interact with or bind to human B7 related protein-1 (B7RP1) and antibodies that bind to and neutralize the function of B7RP1 thereby. The invention also provides pharmaceutical compositions of said antibodies and methods for neutralizing B7RP1 function, and particularly for treating immune disorders (e.g., inappropriate immune response) by administering a pharmaceutically effective amount of anti-B7RP1 antibodies. Methods of detecting the amount of B7RP1 in a sample using anti-B7RP1 antibodies are also provided.
Claims30
An isolated nucleic acid molecule that encodes an antibody or antigen-binding fragment thereof, that binds specifically to human B7RP1, wherein the antibody or antigen-binding fragment thereof comprises a heavy chain variable region and a light chain variable region, wherein the heavy chain variable region comprises any one of SEQ ID NOS:
- 7-14.
An isolated nucleic acid molecule that encodes an antibody or antigen-binding fragment thereof that binds specifically to human B7RP1 comprising:
- a) a heavy chain having a heavy chain variable region comprising SEQ ID NO:
- 7 and a light chain having a light chain variable region comprising SEQ ID NO:
- 1;
- b) a heavy chain having a heavy chain variable region comprising SEQ ID NO:
- 8 and a light chain having a light chain variable region comprising SEQ ID NO:
- 1;
- c) a heavy chain having a heavy chain variable region comprising SEQ ID NO:
- 9 and a light chain having a light chain variable region comprising SEQ ID NO:
- 2;
- d) a heavy chain having a heavy chain variable region comprising SEQ ID NO:
- 10 and a light chain having a light chain variable region comprising SEQ ID NO:
- 3;
- e) a heavy chain having a heavy chain variable region comprising SEQ ID NO:
- 11 and a light chain having a light chain variable region comprising SEQ ID NO:
- 3;
- f) a heavy chain having a heavy chain variable region comprising SEQ ID NO:
- 12 and a light chain having a light chain variable region comprising SEQ ID NO:
- 4;
- g) a heavy chain having a heavy chain variable region comprising SEQ ID NO:
- 13 and a light chain having a light chain variable region comprising SEQ ID NO:
- 5;
- or
- h) a heavy chain having a heavy chain variable region comprising SEQ ID NO:
- 14 and a light chain having a light chain variable region comprising SEQ ID NO:
- 6.
An isolated nucleic acid molecule that encodes an antibody or antigen-binding fragment thereof that binds specifically to human B7RP1, wherein the antibody or antigen-binding fragment thereof comprises a heavy chain and a light chain, wherein the light chain comprises a light chain variable region comprising any one of SEQ ID NOS:
- 1-6.
An isolated nucleic acid molecule that encodes an antibody or antigen-binding fragment thereof that binds specifically to human B7RP1, wherein the antibody or antigen-binding fragment thereof comprises a heavy chain and a light chain, the heavy chain comprising:
- a) a heavy chain CDR1 comprising SEQ ID NO:
- 27, a heavy chain CDR2 comprising SEQ ID NO:
- 28, and a heavy chain CDR3 comprising SEQ ID NO:
- 29;
- b) a heavy chain CDR1 comprising SEQ ID NO:
- 30, a heavy chain CDR2 comprising SEQ ID NO:
- 31, and a heavy chain CDR3 comprising SEQ ID NO:
- 32;
- c) a heavy chain CDR1 comprising SEQ ID NO:
- 27, a heavy chain CDR2 comprising SEQ ID NO:
- 33, and a heavy chain CDR3 comprising SEQ ID NO:
- 34;
- d) a heavy chain CDR1 comprising SEQ ID NO:
- 35, a heavy chain CDR2 comprising SEQ ID NO:
- 36, and a heavy chain CDR3 comprising SEQ ID NO:
- 37;
- e) a heavy chain CDR1 comprising SEQ ID NO:
- 27, a heavy chain CDR2 comprising SEQ ID NO:
- 33, and a heavy chain CDR3 comprising SEQ ID NO:
- 38;
- or
- f) a heavy chain CDR1 comprising SEQ ID NO:
- 35, a heavy chain CDR2 comprising SEQ ID NO:
- 39, and a heavy chain CDR3 comprising SEQ ID NO:
- 40.
An isolated nucleic acid molecule comprising a nucleotide sequence that encodes a peptide comprising any one of SEQ ID NO:
- 1-14, 44-58, and 70-76, or a combination thereof.
An isolated nucleic acid molecule that encodes an antibody or antigen-binding fragment thereof that binds specifically to human B7RP1, wherein the antibody or antigen-binding fragment thereof binds to a region of B7RP1 consisting of SEQ ID NO:
- 67 and does not bind a region of B7RP1 consisting of SEQ ID NO:
- 68.
An isolated nucleic acid molecule that encodes an antibody or an antigen-binding fragment thereof that binds to human B7RP1, wherein the antibody or antigen-binding fragment thereof is competitive with an antibody that comprises a light chain variable region or SEQ ID NO:
- 1 and a heavy chain variable region of SEQ ID NO:
- 8 for binding to human B7RP1.
A vector comprising the nucleic acid molecule of claim 24 or 25.
A host cell comprising the vector of claim 32.
An isolated nucleic acid molecule that encodes an antibody or antigen-binding fragment thereof that binds specifically to human B7RP1, wherein the antibody or antigen-binding fragment thereof comprises any one of SEQ ID NOS:
- 44-58 and 70-76.
Dependencies are inferred from the claim text.