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US 2013/0171693 A1Published application
Process for production of recombinant human growth hormone
AssigneeFerring B.V.
InventorsDov Kanner, Eli Schmell
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Abstract
The subject invention provides an improved process for the production of human growth hormone.
Claims30
A process for the production of human growth hormone (hGH) comprising:
- i. fermenting human growth hormone producing E. coli cells;
- ii. recovering inclusion bodies from the E. coli cells and dissolving the recovered inclusion bodies at an alkaline pH to provide dissolved hGH;
- iii. optionally lyophilizing dissolved hGH;
- and wherein the fermentation is carried out in a culture medium comprising manganese, zinc, cobalt, molybdenum, calcium, copper, and boron as trace elements.
1 more element
A method for reducing the amount of human growth hormone polymeric forms formed during a process for the production of human growth hormone (hGH) comprising:
- i. fermenting human growth hormone producing E. coli cells;
- ii. recovering inclusion bodies from the E. coli cells and dissolving the recovered inclusion bodies at an alkaline pH to provide dissolved hGH;
- iii. optionally lyophilizing dissolved hGH;
- and wherein the fermentation is carried out in a culture medium comprising manganese, zinc, cobalt, molybdenum, calcium, copper, and boron as trace elements.
1 more element
Dependencies are inferred from the claim text.