Sugar-chain modified yeast and method for producing glycoprotein using the same

A mutant yeast which has an ability to produce N-linked sugar chains of Man5GlcNAc2, and further has an ability to produce N-linked sugar chains of GlcNAc1Man5GlcNAc2, wherein the yeast is functionally deficient in an α-1,6-mannosyltransferase gene, an α-1,3 mannosyltransferase gene, and a mannose-1-phosphorylation control gene and an N-acetylglucosaminetransferase I gene;

• and an α-1,2-mannosidase I gene are introduced.

A mutant yeast functionally deficient in a protein-O-mannosyltransferase gene, an α-1,6-mannosyltransferase gene, an α-1,3 mannosyltransferase gene, and a mannose-1-phosphorylation control gene and bearing at least one introduced copy of an α-1,2-mannosidase I gene, wherein the mutant yeast is capable of producing N-linked sugar chains of Man5GlcNAc2 and is capable of suppressing the production of O-linked sugar chains.

A mutant yeast functionally deficient in a protein-O-mannosyltransferase gene, an α-1,6-mannosyltransferase gene, an α-1,3 mannosyltransferase gene, and a mannose-1-phosphorylation control gene and bearing at least one introduced copy of an α-1,2-mannosidase I gene and at least one introduced copy of an N-acetylglucosaminetransferase I gene, wherein the mutant yeast is capable of producing N-linked sugar chains of Man5GlcNAc2 and GlcNAc1Man5GlcNAc2, and is capable of suppressing the production of O-linked sugar chains.