Method of treating mine-influenced water and soil

A method of trans-seasonal bioremedial treating of mine-influenced water and soil (MIWAS) in situ with microorganisms for removal of contaminants containing heavy metals, comprising the steps of:

• a) sampling indigenous microorganisms from the MIWAS site being treated;
• b) identifying indigenous microorganisms that reduce selected heavy metal compounds to be treated in the MIWAS;
• c) propagating at least one of the indigenous microorganisms identified in step b);
• d) reintroducing propagated microorganisms on the soil surface at the MIWAS site together with a source of nutrients and a carbon source for the microorganisms;
• and
• e) burying the reintroduced microorganisms, source of nutrients, and carbon source.

A method of trans-seasonal bioremedial treating of mine-influenced water and soil (MIWAS) in situ with microorganisms for removal of contaminants containing heavy metals or sulpher compounds, comprising the steps of:

• a) sampling indigenous microorganisms from the MIWAS site being treated;
• b) isolating indigenous microorganisms that reduce selected heavy metal compounds to be treated in the MIWAS;
• c) propagating at least one of the indigenous microorganisms isolated in step b);
• and
• d) reintroducing propagated microorganisms subsurface at the MIWAS site together with a source of nutrients and a carbon source for the microorganisms.