US 2013/0171685 A1 · METHOD AND APPARATUS FOR CHARACTERIZING BIOLOGICAL OBJECTS

Abstract

In order to quantitatively characterize biological objects, for example individual cells, a stimulus is applied to a biological object in a contactless fashion. A measurement and a further measurement are performed on the biological object in order to ascertain a response of the biological object to the stimulus, wherein the measurement and the further measurement comprise detecting Raman scattering on and/or in the biological object and/or capturing data using digital holographic microinterferometry (DHMI). The biological object is characterized according to a result of the measurement and is sorted if needed. The stimulus can be applied by means of a laser beam that creates optical tweezers or an optical trap, by means of ultrasonic waves or an electric or magnetic radio frequency field.

Claims33

Dependent claims

A method for characterizing a biological object, the method comprising:

applying a stimulus to the biological object in a contactless fashion;
carrying out plural measurements on the biological object to ascertain a response of the biological object to the stimulus, wherein, to ascertain the response,
a measurement which comprises a detection of Raman scattering is performed on the biological object before application of the stimulus, and
a further measurement which comprises a further detection of Raman scattering is performed on the biological object after or during application of the stimulus;
performing a comparison of results of the measurement and the further measurement;
and
characterizing the biological object as a function of the comparison of the results of the measurements.

4 more elements

An apparatus for characterizing a biological object, comprising:

a device for generating a stimulus on the biological object;
and
a measurement device for performing a measurement on the biological object to ascertain a response of the biological object to the stimulus, the measurement device being configured for data acquisition by means of a detection of Raman scattering on the biological object, the measurement device being configured, for ascertaining the response of the biological object to the stimulus,
to perform a measurement comprising a detection of the Raman scattering before application of the stimulus, and
to perform a further measurement comprising a further detection of the Raman scattering after or during application of the stimulus;
and
an evaluation logic which is configured to compare results of the measurement performed before application of the stimulus and results of the further measurement performed after or during application of the stimulus, and to characterize the biological object as a function of a comparison of the results of the measurement and of the further measurement.

4 more elements

Dependencies are inferred from the claim text.

Method and apparatus for characterizing biological objects

Life of this patent

Method and apparatus for characterizing biological objects

Life of this patent

Abstract

Claims33