Porous membranes having a hydrophilic coating and methods for their preparation and use
AssigneeGeneral Electric Company
InventorsBing Li, David Roger Moore
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Abstract
A modified porous membrane comprising a polymeric hydrophilic coating bonded to a porous membrane is described. The polymeric hydrophilic coatings grafted to the porous membranes comprise, for example, a PEG moiety such as a PEGMA, a PEGDA, or a TMPET, wherein the polymeric hydrophilic coating on the porous membrane decreases non-specific binding of unwanted material to the porous membrane and increases the signal to noise ratio in immunoassays, in vitro diagnostic tests, and point of care tests. Methods of making these modified porous membranes are also disclosed.
Claims25
A nitrocellulose membrane comprising a polymeric hydrophilic coating bonded to the nitrocellulose membrane.
A device for performing an immunoassay that comprises a nitrocellulose membrane comprising a polymeric hydrophilic coating bonded to the nitrocellulose membrane.
A method for preparing a nitrocellulose membrane comprising a polymeric hydrophilic coating bonded to the nitrocellulose membrane, the method comprising:
- a) providing an unmodified nitrocellulose membrane;
- b) immersing the nitrocellulose membrane in an aqueous solution of a hydrophilic compound;
- c) exposing the porous membrane to electron beam (e-beam) radiation;
- and
- d) drying the nitrocellulose membrane, thereby preparing a nitrocellulose membrane comprising a polymeric hydrophilic coating bonded to the nitrocellulose membrane.
A method for improving the sensitivity of an immunoassay that uses a nitrocellulose membrane for immobilization of a biomolecule, wherein the nitrocellulose membrane used in the immunoassay is a nitrocellulose membrane comprising a polymeric hydrophilic coating bonded to the nitrocellulose membrane.
A method for decreasing non-specific binding in an immunoassay that uses a nitrocellulose membrane for immobilization of a biomolecule, wherein the nitrocellulose membrane used in the immunoassay is a nitrocellulose membrane comprising a polymeric hydrophilic coating bonded to the nitrocellulose membrane.
A method for decreasing signal to noise ratio in an immunoassay that uses a nitrocellulose membrane for immobilization of a biomolecule, wherein the nitrocellulose membrane used in the immunoassay is a nitrocellulose membrane comprising a polymeric hydrophilic coating bonded to the nitrocellulose membrane.
Dependencies are inferred from the claim text.