US 2013/0171656 A1 · DETECTION, IDENTIFICATION AND DIFFERENTIATION OF PROTEUS SPECIES USING THE SPACER REGION

Abstract

The present invention relates to new nucleic acid sequences derived from the ITS (Internal Transcribed Spacer) region, between the 16S and 23S ribosomal ribonucleic acid (rRNA) or rRNA genes, to be used for the specific detection and/or identification of Proteus species, in particular of Proteus mirabilis, Proteus vulgaris and/or Proteus penneri, in a biological sample. The present invention relates also to a method for the specific detection and/or identification of Proteus species, in particular Proteus mirabilis, Proteus vulgaris and/or Proteus penneri, using said new nucleic acid sequences derived from the ITS (Internal Transcribed Spacer) region. It relates also to nucleic acid primers to be used for the amplification of said spacer region of Proteus species in a sample.

Claims19

Dependent claims

A set of two polynucleotide probes, said probes being capable of hybridizing specifically to a nucleic acid molecule selected from the group consisting of SEQ ID NOS:

1 to 17, RNA forms of SEQ ID NOS:
1 to 17 in which T is replaced by U, complements of SEQ ID NOS:
1 to 17, complements of the RNA forms of SEQ ID NOS:
1 to 17 in which T is replaced by U, homologs greater than 95% identical to SEQ ID NOS:
1 to 17, homologs greater than 95% identical to RNA forms of SEQ ID NOS:
Ito 17 in which T is replaced by U, and ATACGTGTTATGTGC (SEQ ID NO:
70);
wherein the probes hybridize specifically to the nucleic acid molecule at locations along the nucleic acid molecule such that there are no more than 25 nucleotides of the nucleic acid molecule between said probes.

5 more elements

Dependencies are inferred from the claim text.

Detection, identification and differentiation of proteus species using the spacer region

Life of this patent

Detection, identification and differentiation of proteus species using the spacer region

Life of this patent

Abstract

Claims19