Blocking Reagent and Methods for the Use Thereof
AssigneeOlink AB
InventorsSimon Fredriksson, Bonnie Tran
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Abstract
The present invention relates to the use of a conjugate of a non-analyte-specific binding protein coupled to a nucleic acid as a blocking reagent in a probe-based detection assay, which uses a probe comprising a proteinaceous analyte-binding partner coupled to a nucleic acid domain to detect an analyte in a sample.
Claims31
-38. (canceled)
A method of detecting an analyte in a sample, which method comprises the use of a probe comprising a proteinaceous analyte-binding partner coupled to a nucleic acid domain, wherein a conjugate of a non-analyte-specific binding protein coupled to a nucleic acid is used as a blocking reagent.
A kit for use in a method for detecting an analyte in a sample, said kit comprising:
- (a) a blocking reagent comprising a non-analyte-specific binding protein coupled to a nucleic acid domain;
- and
- (b) at least one set of probes, wherein each probe in a set comprises a proteinaceous analyte-binding domain and a nucleic acid domain.
A method for producing a blocking reagent comprising:
- (a) extracting serum protein from blood;
- (b) preparing a nucleic acid;
- and
- (c) coupling the protein of (a) with the nucleic acid of (b),
- wherein said serum protein is bulk IgG or serum albumin.
Dependencies are inferred from the claim text.