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US 2013/0171630 A1Published application
Methods of using telomeres as markers for aging
AssigneeQiagen GmbH
InventorJames QIN
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Abstract
The invention relates to a simple, reproducible, fast, and accurate method of quantifying and measuring telomeres in a clinical sample. The invention further relates to kits comprising premixed and optimized buffers, DNA polymerase, primers, and instructions for the detection of telomere length and quantities. Also envisioned are complete kits further including instrumentalities for the detection of telomere length and quantities.
Claims38
A method of measuring a repeating nucleotide sequence in a telomere region comprising the steps of:
- (a) extracting and purifying at least one DNA sample or target DNA template from a subject specimen;
- (b) amplifying the DNA sample in a reaction mixture at high denaturing and annealing temperatures;
- and
- (c) detecting the amplified DNA in the presence of a labeled probe,
- wherein the entire process is completed in under one hour.
2 more elements
Dependencies are inferred from the claim text.