Back to results
US 2013/0171622 A1Published application
Compositions and methods for detecting viral infection using direct-label fluorescence in situ hybridization
AssigneeAbbott Laboratories
InventorsKa-Cheung X. Luk, John R. Hackett, JR., Ekaterina Pestova
Life of this patent
Estimating the term…
Abstract
The present invention relates generally to assays for the detection of viral infection and/or prognosis of viral infection and associated disease states. In particular, the invention relates to directly labeled viral-related nucleic acids having significant diagnostic, prognostic, and screening utilities and methods of using the same.
Claims25
A method of diagnosing a viral infection in a subject comprising:
- (a) contacting a sample from said subject with a directly-labeled nucleic acid composition capable of hybridizing to a viral genomic sequence, wherein said viral genome is 5 kb-10 kb in length,
- (b) washing said contacted sample to remove un-hybridized directly-labeled nucleic acid composition;
- and
- (c) assaying said sample for the presence of the directly-labeled nucleic acid composition;
- wherein the presence of the directly-labeled nucleic acid composition is indicative of a viral infection in said subject.
2 more elements
A method of diagnosing a predisposition to cancer in a subject comprising:
- (a) contacting a sample from said subject with a directly-labeled nucleic acid composition capable of hybridizing to a viral genomic sequence, wherein said viral genome is 5 kb-10 kb in length,
- (b) washing said contacted sample to remove un-hybridized directly-labeled nucleic acid composition;
- and
- (c) assaying said sample for the presence of the directly-labeled nucleic acid composition;
- wherein the presence of the directly-labeled nucleic acid composition is indicative of a predisposition to cancer in said subject.
2 more elements
A method of discriminating between cells expressing viral RNA and cells comprising episomal viral DNA, comprising:
- (a) contacting a first sample from said subject with a directly-labeled nucleic acid composition capable of hybridizing to a viral genomic sequence, wherein said viral genome is 5 kb-10 kb in length,
- (b) washing said contacted sample to remove un-hybridized directly-labeled nucleic acid composition;
- and
- (c) assaying said sample for a signal indicative of the presence of the directly-labeled nucleic acid composition;
- (d) contacting a second sample from said subject with a directly-labeled nucleic acid composition capable of hybridizing to a viral genomic sequence, wherein said viral genome is 5 kb-10 kb in length and said second sample has been pre-treated with RNase;
- (e) washing said contacted sample to remove un-hybridized directly-labeled nucleic acid composition;
- and
- (f) assaying said sample for the presence of a signal indicative of the directly-labeled nucleic acid composition;
- and
- (g) comparing the signal obtained in step (c) with the signal obtained in (f);
- wherein when a diffuse staining pattern of the directly-labeled nucleic acid composition in step (c) is contrasted with a punctate staining pattern of the directly-labeled nucleic acid composition in step (f), such a contrast is indicative of virus integrated into the host genome that is actively expressing viral RNA;
- and wherein the presence of a diffuse staining pattern in both (c) and (f) is indicative of the presence of episomal viral DNA.
9 more elements
A method of diagnosing a predisposition to cancer in a subject comprising:
- (a) contacting a sample from said subject with a directly-labeled nucleic acid composition capable of hybridizing to a viral genomic sequence, wherein said viral genome is 5 kb-10 kb in length,
- (b) washing said contacted sample to remove un-hybridized directly-labeled nucleic acid composition;
- (c) simultaneously or sequentially contacting the sample with a nucleic acid composition capable of hybridizing to a chromosomal marker;
- and
- (d) assaying said sample for the presence of the directly-labeled nucleic acid composition and the nucleic acid composition capable of hybridizing to a chromosomal marker;
- wherein the presence of the directly-labeled nucleic acid composition and an abnormal signal from the nucleic acid composition capable of hybridizing to a chromosomal marker is indicative of a predisposition to cancer in said subject.
3 more elements
Dependencies are inferred from the claim text.