Selective lysis of cells
AssigneeBiocartis NV
InventorsBart Edward Jozef Gusta Van Meerbergen, Oana Mihaela Piciu, Ron Gill, Kristiane Anne Schmidt, Sieglinde Neerken, Marc Wilhelmus Gijsbert Ponjee, Zeynep Seflek, Roel Penterman, Paul Arnold Van De Wiel
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Abstract
Methods and devices for the selective lysis of cells in a sample comprising micro-organisms such as bacteria are provided. The selective lysis is obtained by incubating the sample in a non-ionic detergent under alkaline conditions.
Claims17
1-15. (canceled)
A method for the selective lysis of eukaryotic cells within a sample containing or suspected to contain a micro-organism, the method comprising the steps of:
- providing the sample containing or suspected to contain the micro-organism;
- adding a non-ionic detergent and a buffer to the sample to obtain a solution with a pH of around 9.5 or higher;
- and
- incubating the solution for a time period sufficiently long to lyse eukaryotic cells in the sample.
A device for the detection of micro-organisms in a sample, comprising:
- a lysis chamber for accepting a sample fluid with a volume below 40 ml;
- a first reservoir comprising an alkaline buffer with a pH of 9.5 or higher and comprising a non-ionic detergent, the first reservoir being connected to the lysis chamber;
- a second reservoir comprising a non-ionic detergent, the second reservoir being connected to the lysis chamber;
- a filter connected to the lysis chamber for filtering the sample fluid after lysis, the filter having a pore size which retains bacteria on the filter, and
- a detection chamber for assaying the presence of DNA in the bacteria.
Dependencies are inferred from the claim text.