Sample preparation device for maldi and sample preparation method

A sample preparation device for preparing a sample to be analyzed using a matrix assisted laser desorption/ionization method by forming through vacuum vapor deposition, at least on a substance to be analyzed on a sample substrate to which the substance to be analyzed is pasted, a matrix film where two types of matrix substances A and B (here, the transmittance TAa of a single substance film of the matrix substance A is higher than the transmittance TBb of a single substance film of the matrix substance B) are mixed, comprising:

• two vapor deposition sources for independently heating said two types of matrix substances A and B within a vacuum container so that the matrix substances A and B are vapor deposited;
• a sample substrate support unit for supporting the sample substrate so that the substance to be analyzed on said sample substrate faces both of said two vapor deposition sources;
• a light amount measurement unit made up of a light source, which is provided within said vacuum container and diagonally irradiates the matrix film vapor deposited on said sample substrate with light for measurement, and a light detector for detecting the amount of light for measurement that has diagonally transmitted through or has been reflected from the matrix film vapor deposited on said sample substrate;
• and
• an adhesion prevention means for preventing the matrix substances A and B that have flown off from said two vapor deposition sources from adhering to said light amount measurement unit, characterized in that
• said light amount measurement unit has the light source using light with a certain wavelength that transmits through the matrix substance A, and at the same time is absorbed by the matrix substance B.