Stimuli Responsive Nanofibers
AssigneeInnovative Surface Technologies, Inc.
InventorsTahmina Naqvi, Jie Wen, Patrick Guire
Life of this patent
Estimating the term…
Abstract
A stimuli responsive nanofiber that includes a stimuli responsive polymer, such as a thermally responsive polymer, and a cross-linking agent having at least two latent reactive activatable groups. The nanofiber may also include a biologically active material or a functional polymer. The stimuli responsive nanofiber can be used to modify the surface of a substrate. When the nanofiber includes a thermally responsive polymer, the physical properties of the surface can be controlled by controlling the temperature of the system, thus controlling the ability of the surface to bind to a biologically active material of interest.
Claims19
-52. (canceled)
A method for preparing a cell culture article, the method comprising steps of:
- (a) contacting a surface of the cell culture article with a coating composition, the coating composition comprising a thermally responsive polymer that binds and releases biological material by application or removal of heat, and a crosslinking agent having at least two latent reactive groups;
- and
- (b) treating the coating composition to activate latent reactive groups of the crosslinking agent, thereby coupling the stimuli responsive polymer to the surface of the cell culture article in a manner in which at least some of the latent reactive groups remain in an inactive state.
A method for preparing a cell culture article, the method comprising steps of:
- (a) contacting a surface of the cell culture article with a crosslinking agent having at least two latent reactive groups;
- (b) treating the crosslinking agent to activate less than all of the latent reactive groups of the crosslinking agent, thereby coupling the crosslinking agent to the surface of the cell culture article and allowing at least some of the latent reactive groups to remain in an inactive state;
- (c) contacting the surface of the cell culture article with a thermally responsive polymer that binds and releases biological material by application or removal of heat;
- (d) treating the surface of the cell culture article to activate latent reactive groups which are present in an inactive state subsequent to step (b), thereby coupling the thermally responsive polymer to the cell culture article surface.
Dependencies are inferred from the claim text.