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US 2013/0171319 A1Published application
Method for producing a large region duplication of aspergillus chromosome
AssigneeKikkoman Corporation
InventorTadashi Takahashi
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AssigneeKikkoman Corporation
InventorTadashi Takahashi
Estimating the term…
Disclosed is a technique which is for duplicating across a wide region any large region on a chromosome of a fungus belonging to Aspergillus strain, and which enables the stable and systematic acquisition of a previously un-acquirable fungus belonging to the Aspergillus strain having novel traits. Disclosed is a transformant wherein a transformation marker gene sequence is arranged on the outside of terminals (5′, 3′) of a region so as to sandwich an arbitrary region of a chromosome, and chromosome duplication has taken place by means of homologous recombination after double strand breakage generated in a homologous region inside the gene. Also disclosed is a method for duplicating an arbitrary region of a chromosome of a fungus belonging to Aspergillus strain by culturing said transformant.
A transformant which is characterized by being constructed by integrating a transformation marker gene with deficiency in a terminal part at one of the 5′ or 3′ end of its coding region into the outside of one of the 5′ or 3′ end of a targeted region to be duplicated on a chromosome of a fungus belonging to Aspergillus, and the transformation marker gene with deficiency in a terminal part at the other end of its coding region into the outside of the other end of the targeted region, so that the targeted region is sandwiched between the transformation marker genes with the deficiency in the terminal part at the 5′ and 3′ ends, respectively, wherein a sequence adjacent to the deficiency of each integrated transformation marker gene is located at an opposite side of the targeted region.
Dependencies are inferred from the claim text.