Method
AssigneeDupont Nutrition Biosciences Aps
InventorMai Faurschou Isaksen
Life of this patent
Estimating the term…
Abstract
The present invention relates to a method for identifying a pepsin resistant alpha amylase enzyme for use in a feed supplement comprising: i) providing an alpha amylase enzyme; ii) admixing said alpha amylase with corn based feed and buffer solution comprising a pepsin concentration of 9000 U/ml at pH 3, 40° C., 500 rpm for at least 120 minutes and analysing alpha amylase activity on said alpha amylase compared to a control sample; wherein said control sample differs in that no pepsin is present during incubation; and iii) selecting an alpha amylase enzyme which substantially maintains alpha amylase activity under the assay conditions; feed supplements and feedstuffs comprising a pepsin resistant alpha amylase and the use of pepsin resistant alpha amylases in feed.
Claims26
A method for identifying a pepsin resistant alpha amylase enzyme for use in a feed supplement comprising:
- i) providing an alpha amylase enzyme;
- ii) admixing said alpha amylase enzyme with corn based feed and pepsin, incubating at pH 3, and analyzing alpha amylase activity of said alpha amylase enzyme compared to a control sample, wherein said control sample differs in that no pepsin is present during the incubation at pH 3;
- and
- iii) selecting the alpha amylase enzyme from one or more alpha amylase enzymes that substantially maintains the alpha amylase activity under assay conditions.
6-7. (canceled)
A method for preparing a feed supplement for a monogastric animal comprising admixing a pepsin resistant alpha amylase with at least one physiologically acceptable carrier selected from the group consisting of maltodextrin, limestone (calcium carbonate), cyclodextrin, wheat or a wheat component, sucrose, starch, Na2SO4, Talc, PVA, sorbitol, benzoate, sorbiate, glycerol, sucrose, propylene glycol, 1,3-propane diol, glucose, parabens, sodium chloride, citrate, acetate, phosphate, calcium, metabisulfite, formate and mixtures thereof.
A feed supplement for monogastric animals comprising a pepsin resistant alpha amylase and at least one physiologically acceptable carrier selected from the group consisting of maltodextrin, limestone (calcium carbonate), cyclodextrin, wheat or a wheat component, sucrose, starch, Na2SO4, Talc, PVA, sorbitol, benzoate, sorbiate, glycerol, sucrose, propylene glycol, 1,3-propane diol, glucose, parabens, sodium chloride, citrate, acetate, phosphate, calcium, metabisulfite, formate and mixtures thereof.
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Dependencies are inferred from the claim text.