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US 2013/0171241 A1Published application
Liposomes with lipids having an advantageous pka-value for rna delivery
AssigneeNovartis AG
InventorAndrew Geall
Life of this patent
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Abstract
RNA encoding an immunogen is delivered in a liposome for the purposes of immunisation. The liposome includes lipids which have a pKa in the range of 5.0 to 7.6 and, preferably, a tertiary amine. These liposomes can have essentially neutral surface charge at physiological pH and are effective for immunisation.
Claims14
A liposome having a lipid bilayer encapsulating an aqueous core, wherein:
- (i) the lipid bilayer comprises a lipid having a pKa in the range of 5.0 to 7.6;
- and (ii) the aqueous core includes a RNA which encodes an immunogen.
A process for preparing a RNA-containing liposome, comprising steps during liposome formation of:
- (a) mixing RNA with a lipid at a pH which is below the lipid's pKa but is above 4.5;
- then (b) increasing the pH to be above the lipid's pKa.
Dependencies are inferred from the claim text.